John Dubé, Ph.D.

Photo of John Dube
Program Director, MA Biomedical Studies, and Associate Professor of Biology
Buhl - 122B

Hometown:  Winchester, VA


Skeletal muscle biology, cardiometabolic disease, and mitochondrial function in human health and disease 

  • National Institutes of Health Loan Repayment Program Recipient (2008)
  • Andreacci JL, Robertson RJ, Dubé JJ, Aaron DJ, Balasekaran G, Arslanian SA. Comparison of maximal oxygen consumption between black and white prepubertal and pubertal children. Pediatr Res. 2004 Nov;56(5):706-13. PubMed PMID: 15319460.
  • Commerford SR, Peng L, Dubé JJ, O'Doherty RM. In vivo regulation of SREBP-1c in skeletal muscle: effects of nutritional status, glucose, insulin, and leptin. Am J Physiol Regul Integr Comp Physiol. 2004 Jul;287(1):R218-27. PubMed PMID: 15001432.
  • Goss FL, Robertson RJ, Swan PD, Harris GS, Trone GA, Dubé JJ, Utter AC. Comparison of measured and predicted residual lung volume in determining body composition of collegiate wrestlers. J Strength Cond Res. 2004 May;18(2):281-5. PubMed PMID: 15141998.
  • Andreacci JL, Robertson RJ, Dubé JJ, Aaron DJ, Dixon CB, Arslanian SA. Comparison of maximal oxygen consumption between obese black and white adolescents. Pediatr Res. 2005 Sep;58(3):478-82. PubMed PMID: 16148060.
  • Robertson RJ, Goss FL, Andreacci JL, Dubé JJ, Rutkowski JJ, Frazee KM, Aaron DJ, Metz KF, Kowallis RA, Snee BM. Validation of the Children's OMNI-Resistance Exercise Scale of perceived exertion. Med Sci Sports Exerc. 2005 May;37(5):819-26. PubMed PMID: 15870636.
  • Robertson RJ, Goss FL, Andreacci JL, Dubé JJ, Rutkowski JJ, Snee BM, Kowallis RA, Crawford K, Aaron DJ, Metz KF. Validation of the children's OMNI RPE scale for stepping exercise. Med Sci Sports Exerc. 2005 Feb;37(2):290-8. PubMed PMID: 15692326.
  • Bhatt BA, Dubé JJ, Dedousis N, Reider JA, O'Doherty RM. Diet-induced obesity and acute hyperlipidemia reduce IkappaBalpha levels in rat skeletal muscle in a fiber-type dependent manner. Am J Physiol Regul Integr Comp Physiol. 2006 Jan;290(1):R233-40. PubMed PMID: 16081881.
  • Dubé JJ, Bhatt BA, Dedousis N, Bonen A, O'Doherty RM. Leptin, skeletal muscle lipids, and lipid-induced insulin resistance. Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R642-50. Epub 2007/05/11. doi: 10.1152/ajpregu.00133.2007. PubMed PMID: 17491114.
  • Amati F, Dubé JJ, Shay C, Goodpaster BH. Separate and combined effects of exercise training and weight loss on exercise efficiency and substrate oxidation. J Appl Physiol (1985). 2008 Sep;105(3):825-31. PMCID:PMC2536819.
  • Dubé JJ, Amati F, Stefanovic-Racic M, Toledo FG, Sauers SE, Goodpaster BH. Exercise-induced alterations in intramyocellular lipids and insulin resistance: the athlete's paradox revisited. Am J Physiol Endocrinol Metab. 2008 May;294(5):E882-8. PMCID:PMC3804891
  • Amati F, Dubé JJ, Coen PM, Stefanovic-Racic M, Toledo FG, Goodpaster BH. Physical inactivity and obesity underlie the insulin resistance of aging. Diabetes Care. 2009;32(8):1547-9. PMCID:PMC2713647.
  • Amati F, Dubé JJ, Stefanovic-Racic M, Toledo FG, Goodpaster BH. Improvements in insulin sensitivity are blunted by subclinical hypothyroidism. Med Sci Sports Exerc. 2009 Feb;41(2):265-9. PubMed PMID: 19127201.
  • Chomentowski P, Dubé JJ, Amati F, Stefanovic-Racic M, Zhu S, Toledo FG, Goodpaster BH. Moderate exercise attenuates the loss of skeletal muscle mass that occurs with intentional caloric restriction-induced weight loss in older, overweight to obese adults. J Gerontol A Biol Sci Med Sci. 2009 May;64(5):575-80. PMCID:PMC2800807.
  • Gonzalez-Pertusa J, Dubé J, Valle SR, Rosa TC, Takane KK, Mellado-Gil JM, Perdomo G, Vasavada RC, García-Ocaña. Novel Proapoptotic Effect of Hepatocyte Growth Factor: Synergy with Palmitate to Cause Pancreatic ß-Cell Apoptosis. Endocrinology. 2010 April; 151(4): 1487–1498. PMCID:PMC2850223.
  • Coen PM, Dubé JJ, Amati F, Stefanovic-Racic M, Ferrell RE, Toledo FG, Goodpaster BH. Insulin resistance is associated with higher intramyocellular triglycerides in type I but not type II myocytes concomitant with higher ceramide content. Diabetes. 2010;59(1):80-8. PMCID:PMC2797948.
  • Huang W, Metlakunta A, Dedousis N, Zhang P, Sipula I, Dubé JJ, Scott DK, O'Doherty RM. Depletion of liver Kupffer cells prevents the development of diet-induced hepatic steatosis and insulin resistance. Diabetes. 2010;59(2):347-57. PMCID:PMC2809951.
  • Amati F, Dubé JJ, Alvarez-Carnero E, Edreira MM, Chomentowski P, Coen PM, Switzer GE, Bickel PE, Stefanovic-Racic M, Toledo FG, Goodpaster BH. Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes? Diabetes. 2011;60(10):2588-97. PMCID:PMC3178290.
  • Dubé JJ, Amati F, Toledo FG, Stefanovic-Racic M, Rossi A, Coen P, Coen P and Goodpaster BH. Effects of weight loss and exercise on insulin resistance, and intramyocellular triacylglycerol, diacylglycerol and ceramide. Diabetologia. 2011;54(5):1147-56. PMCID:PMC3804898.
  • Amati F, Pennant M, Azuma K, Dubé JJ, Toledo FG, Rossi AP, Kelley DE, Goodpaster BH Lower thigh subcutaneous and higher visceral abdominal adipose tissue content both contribute to insulin resistance. Obesity (Silver Spring, Md). 2012;20(5):1115-7. PMID: 22262160.
  • Dubé JJ, Allison KF, Rousson V, Goodpaster BH, Amati F. Exercise dose and insulin sensitivity: relevance for diabetes prevention. Med Sci Sports Exerc. 2012 May;44(5):793-9. PMCID: PMC3328621.
  • Finlin BS, Varma V, Nolen GT, Dubé J, Starnes CP, Rasouli N, Kern PA, Peterson CA. DHA reduces the atrophy-associated Fn14 protein in differentiated myotubes during coculture with macrophages. J Nutr Biochem. 2012 Aug;23(8):885-91. PMCID: PMC3223324
  • Coen PM, Hames KC, Leachman EM, Delany JP, Ritov VB, Menshikova EV, Dubé JJ, Toledo FG, Stefanovic-Racic M, Goodpaster BH. Reduced skeletal muscle oxidative capacity and elevated ceramide but not diacylglycerol content in severe obesity. Obesity (Silver Spring, Md). 2013. PMCID:PMC4136513.
  • Liang H, Tantiwong P, Sriwijitkamol A, Shanmugasundaram K, Mohan S, Espinoza S, Dubé JJ, Defronzo RA, Musi N. Effect of a sustained reduction in plasma free fatty Acid concentration on insulin signaling and inflammation in skeletal muscle from human subjects. J Physiol. 2013; 591(pt11):2897-909. PMCID: PMC3690693.
  • Avin KG, Coen PM, Huang W, Stolz DB, Sowa GA, Dubé JJ, Goodpaster BH, O’Doherty RM, Ambrosio F. Skeletal muscle as a regulator of the longevity protein, Klotho. Front Physiol. 2014 Jun 17;5:189. PMCID:PMC4060456.
  • DeLany JP, Dubé JJ, Standley RA, Distefano G, Goodpaster BH, Stefanovic-Racic M, Coen PM, Toledo FG. Racial Differences In Peripheral Insulin Sensitivity And Mitochondrial Capacity In The Absence Of Obesity. J Clin Endocrinol Metab. 2014 Aug 8:jc20142512.
  • Dubé JJ, Coen PM,DiStefano G, Chacon AC, Helbling NL, Desimone ME, Stafanovic-Racic M, Hames KC, Despines AA, Toledo FGS, Goodpaster BH. Effects of acute lipid overload on skeletal muscle insulin resistance, metabolic flexibility, and mitochondrial performance. Am J Physiol Endocrinol Metab. 2014. Dec 15;307(12):E1117-24. PMCID:pending
  • Dubé JJ, Sitnick MT, Schoiswohl G, Wills RC, Basantani MK, Cai L, Kershaw EE. Adipose triglyceride lipase (ATGL) deletion from adipocytes, but not skeletal myocytes impairs acute exercise performance in mice. Am J Physiol Endocrinol Metab. 2014. May 15; 308(10): E879-90. PMCID:pending
  • Menshikova EV, Ritov VB, Dubé JJ, Amati F, Stefanovic-Racic M, Toledo FGS, Coem PM, Goodpaster BH. Calorie Restriction-induced Weight Loss and Exercise Have Difference Effects on Skeletal Muscle Mitochondria Despite Similar Effects on Insulin-Sensitivity. J Gerontol A Biol Sci Med Sci. 2017 Feb 2 [Epub ahead of print]. PMCID pending.
  • Chow LS, Mashek DG, Wang Q, Shepherd SO, Goodpaster BH, Dubé JJ. Effect of acute physiological free fatty acid elevation in the context of hyperinsulinemia on fiber type specific IMCL accumulation. J Appl Physiol. 2017 Apr 27 [Epub ahead of print]. PMCID pending.
  • Shepherd SO, Strauss JA, Wang Q, Dubé JJ, Goodpaster BH, Mashek DG, Chow LS. Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure. J Physiol. In press.
  • “Self-induced Sensorimotor Sports & Recreation Injuries Among Adolescents”. American College of Sports Medicine Annual Meeting, Baltimore, MD. June 2001
  • “Comparison of Substrate Utilization During Treadmill Exercise in Children Varying in BMI”. American College of Sports Medicine Annual Meeting, St. Louis, MO. June 2002
  • “Comparison of anaerobic threshold using NIRS and ventilation methods in males and females”. American College of Sports Medicine Annual Meeting, San Francisco, CA. May 2003
  • “Hyperleptinemia Prevents Acute Hyperlipidemia-Induced Insulin Resistance”. American Diabetes Association 65th Scientific Sessions, San Diego, CA. June 2005
  • “Separate effects of exercise and weight-loss on insulin resistance and cardiometabolic risk in older obese adults’. American Diabetes Association 67th Scientific Sessions, Chicago. June 2007
  • “Substrate Availability and Utilization Following Exercise Training in Obese Older Adults”. American College of Sports Medicine 55th Annual Meeting. May 2008
  • “Lipid-induced insulin resistance: Good news, bad news”. University of Kentucky Graduate Center for Nutritional Sciences seminar. January 2010
  • “High oxidative muscle is not protected from acute lipid-induced insulin resistance”, Oral presentation American Diabetes Association Scientific Sessions. June 2012
  • “Impaired Malonyl-CoA Synthesis in Response to Insulin and Metabolic Inflexibility in Obesity and Type 2 Diabetes”. AC Chacon, EV Menshikova, JP DeLany, VB Ritov and JJ Dubé. Poster presentation American Diabetes Association Scientific Sessions. June 2012
  • Invited speaker. American Diabetes Association 73rd Scientific Sessions. Title “Exercise and Lipid intermediates—What exercise has revealed about lipotoxicity”. June 2013